Custom Peptides
From mg to low gr scale. For larger amounts, please inquire at request@invatispeptides.com
For crude custom peptides, 4 weeks are usually required, for purified custom peptides 4-6 weeks. Delivery times may vary depending on number of peptides, complexity, amount, and purity.
No, we only charge for custom peptides that are delivered.
No, we produce custom peptides in our own facility in Tübingen, Germany. In rare cases we outsource to partners, who are also situated in Germany.
Please provide your peptide sequences (single or 3-letter code), C- and N-terminal functions, counterion (TFA, chloride or acetate) amount (mg), purity (%), invoicing address and delivery address.
Yes, please reach out and we will discuss your specific custom peptide project with you.
Write to request@intavispeptides.com
If used up rather quickly within weeks, mostly refrigerated temperatures are fine, but -20°C is preferred. When stored for months and years, store them at -20°C or ideally at -80°C. After longer storage, ideally test purity through analytical HPLC-MS.
Peptide Libraries
We start to call it libraries from 48 peptides onwards, but this term is a vague definition.
We can essentially any scale in the mg range in library format, but the regular amount would be 1-2 mg per peptide. We use automated synthesizers, that can generate many peptides in parallel in that scale.
Yes, we surely can synthesize peptide libraries with chemical modifications.
Very often, peptides ordered in library formats are used in early discovery stage and thus, very high purities may not be required. They may often be used as crude material without purification or at a guaranteed purity of above 70%. However, also higher purities are possible.
When screening for MHC class I T cell epitopes, often 9-11mer peptides are used, which should be synthesized with a C-terminal acid. For MHC class II T cell epitope and B cell epitope screenings, C-terminal amides work as well and are more robust than acids.
Peptides are delivered as freeze-dried powder in separate vials, except for different arrangements discussed prior to the synthesis.
Yes, we can weigh each individual peptide for you to gain precision.
Peptide Arrays - SPOT
We can synthesize up to 600 peptide spots per cellulose membrane.
The standard format is 100x150mm with 5 spots per cm² and spot diameter of approx. 2-3 mm.
Yes, they are covalently bound through the C-terminus via an amide bond to amine-functionalized membrane.
Per spot, there are about 5-10 nmol peptide immobilized on the membrane.
SPOT peptide arrays are commonly used in epitope or kinase mapping, protein interaction assays, binding optimization studies and immune screenings.
Yes, they can be reused to a certain extent, but be aware that this may have an impact on the result of your assay. A membrane stripping-protocol can be requested from us.
Yes, they can be scanned by those and are BLOT-compatible.
CelluSpot
The CelluSpot peptide array has a standard microscope slide format and is coated with inert white background foil.
The distance is about 1.2 mm with a spot diameter of 0.8 mm.
You can get up to 768 peptide spots per microscope slide.
Yes, you can have dozens of identical peptide arrays for parallel screening.
Yes, the peptides are immobilized via their C-terminus.
CelluSpot peptide arrays are often used to screen human sera, cell lysates, to characterize antibodies, enzyme substrates or to determine sequence specificities of interaction partners.
Common detection methods are autoradiography, chemieluminescence, chromogenic substrate reactions, dedicated fluorescent dyes.
Peptide Nucleic acids (PNAs)
We recommend not to exceed 20 bases if possible, because the efficiency of the synthesis starts to drastically decrease upon further elongation. Longer sequences can increase the costs.
Yes, this is possible. We can attach several amino acids to your PNA, such as e.g. short CPP sequences.
Yes, this is in general possible through either coupling to the N-terminus of the PNA or through the introduction of C-terminal or N-terminal Lysine, which can be modified at the side-chain amino function.
We offer standard purities of >70% and >90% UV-purity after HPLC purification.
The standard scales are either 30 nmol, 100 nmol or 1 µmol. For larger amounts, please contact our staff via request@intavispeptides.com
The nucleobases are connected via a peptide backbone, that replaced the phosphate backbone.
Yes, PNA has a strong binding-affinity to DNA.
Order your peptides
Click order and a form will pop-up so you can enter your sequence directly. Alternatively upload a document listing your peptides or conjugates.